Why Is qPCR Still Positive After Biocide Treatment
Why Is qPCR Still Positive After Biocide Treatment? | MICBUSTERS Post-biocide molecular monitoring Why Is qPCR Still Positive After Biocide Treatment? Standard DNA-based qPCR can detect DNA from viable, low-activity, dormant, injured and recently inactivated cells. It can also detect extracellular DNA. A positive result after treatment therefore shows that the selected target DNA is […]
low atp high qpcr results mic
ATP Is Low but qPCR Is High: How Is That Possible? | MICBUSTERS Interpreting apparently conflicting microbial results ATP Is Low but qPCR Is High: How Is That Possible? Low ATP and high qPCR can both be analytically valid. The results are not direct equivalents: ATP assays quantify ATP recovered under a defined procedure, whereas […]
oilfield microbiology sample holding time transport
How Quickly Should an Oilfield Water Sample Be Tested? | MICBUSTERS Produced-water sampling, transport and preservation How Quickly Should an Oilfield Water Sample Be Tested? For culture and MPN testing, an oilfield water sample should be inoculated as close to collection as safely possible—ideally within minutes. Once the sample leaves the system, pressure, dissolved gases, […]
can pbs kill salt adapted oilfield microorganisms
Can PBS Kill Salt-Adapted Oilfield Microorganisms? | MICBUSTERS Osmotic shock and oilfield microbial recovery Can PBS Kill Salt-Adapted Oilfield Microorganisms? PBS is not inherently bactericidal, but standard PBS can be a severe ionic and salinity downshift for microorganisms adapted to hypersaline produced water. Depending on the organism and exposure conditions, this can reduce survival, prolong […]
pbs vs saline vs formation water oilfield microbiology
PBS vs Saline vs Formation Water for Oilfield Microbiology | MICBUSTERS Dilution-fluid selection for oilfield microbiology PBS vs Saline vs Formation Water for Oilfield Microbiology PBS, simple saline and sterile synthetic formation water are not interchangeable dilution fluids. PBS stabilizes pH, saline provides a simple salt background, and synthetic formation water can reduce osmotic and […]
srb bottle stays clear false negative postgate b
Why Does an SRB Bottle Stay Clear Even When Sulfide Is Present? | MICBUSTERS False-negative SRB culture troubleshooting Why Does an SRB Bottle Stay Clear Even When Sulfide Is Present? A clear Modified Postgate B or API bottle does not automatically prove that sulfate-reducing microorganisms are absent. The sulfide may not have entered or remained […]
rust black solids interfere srb test bottles
Can Rust or Black Solids Interfere with SRB Test Bottles? | MICBUSTERS SRB culture troubleshooting for solid and dirty samples Can Rust or Black Solids Interfere with SRB Test Bottles? Yes. Rust, pig debris, sludge and black corrosion products can obscure or imitate the visible black endpoint in an SRB culture bottle. Pre-existing iron-sulfide particles […]
sulfide false positive srb test blackening
Can Sulfide Cause a False-Positive SRB Test? | MICBUSTERS SRB bottle troubleshooting for oil & gas Can Sulfide Cause a False-Positive SRB Test? Yes. Sulfide already present in a produced-water or deposit sample can react with iron in an SRB growth medium and cause immediate blackening without new microbial growth. Published: 6 July 2026 Reading […]
how to detect mic sampling plan tests standards
How to Detect MIC: Sampling Plan, Tests and Standards | MICBUSTERS Practical MIC investigation guide How to Detect MIC: A Practical Sampling Plan, Tests and Standards Microbiologically influenced corrosion cannot be confirmed from one black culture bottle, one qPCR result or one pit photograph. A defensible investigation starts with the operating question, protects the evidence […]
mic microbiology srb sob irb what to measure
MIC Microbiology: SRB, SOB and IRB Explained | MICBUSTERS Functional microbiology for MIC monitoring MIC Microbiology: SRB, SOB and IRB — What Should You Measure and Why? “SRB,” “SOB” and “IRB” are commonly requested in microbiologically influenced corrosion testing. They are not single organisms and they do not represent one corrosion mechanism. A useful monitoring […]