qPCR Below LOQ: Symbols & Non-Detects | MICBUSTERS
MICBUSTERS · qPCR quality How to Interpret qPCR Results Near the Detection Limit A sample can be clearly positive for a selected target even when there is too little DNA for a reliable numerical concentration. This is why detection and quantification have different limits. Practical field guide · MICBUSTERS Technical Team Direct answer “Detected below […]
Gene Copies Are Not Cells: 16S & Halanaerobium | MICBUSTERS
MICBUSTERS · qPCR quality Gene Copies Are Not the Same as Cells in qPCR A certificate showing 10,000 gene copies per millilitre is reporting an amount of a selected DNA sequence. It is not saying that 10,000 living microbes were present. Practical field guide · MICBUSTERS Technical Team Direct answer Keep the measurement in its […]
Industrial qPCR Inhibition: Causes & Fixes | MICBUSTERS
MICBUSTERS · qPCR quality qPCR Inhibition in Industrial Samples: Causes and Practical Fixes A non-detect in oily, iron-rich or high-salt material is only useful when the sample has passed its quality checks. Problems can start before PCR, during DNA isolation or inside the reaction. Practical field guide · MICBUSTERS Technical Team Direct answer Test the […]
qPCR Accuracy for MIC: LOD, LOQ & Precision | MICBUSTERS
MICBUSTERS · qPCR quality How Accurate Is qPCR for MIC Monitoring? qPCR can follow a well-defined microbial target over a wide concentration range. The defensible question is how reliably the complete sampling and extraction workflow measures that target in your matrix. Practical field guide · MICBUSTERS Technical Team Direct answer Accurate for a validated target; […]